Today we are going to instruct a completely automated robotic cloud lab to grab a genetically modified strain of bacteria from a library of common reagents, innoculate some bacterial growth media and finally watch how that culture grows over 8 hours by seeing how the bacteria scatter 600nm light.
I was thinking that when writing a protocol with Autoprotocol it would be really useful to say “Give me a plate of a drug that covers a dose range from 10nM to 100nM”. It turned out that this was a little bit more complex than I had originally thought.
Phew it’s been a while! I’ve been busy, but in all honesty I haven’t done a Transcriptic blog recently because I’ve been doing a lot of repetition trying to optimise plating bacteria. There’s a lot to write about, but I think it would be best for both you and I if I try and keep it short. In this post I want to cover doing some rough analysis of early data from my burden assay project on Transcriptic.